Table 1.

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidGenotype or descriptionSource (reference)
E. coli K-12
 MC1061 hsdR hsdM+ araD139 Δ(ara-leu)7679 Δlac(IPOZYA) galU galK rpsL E. W. Nester (28)
 MS8MC1061rnh::cat H. Ohmori (20)
 SN1216MC1061cpxR::Kmr This study
 SN1044MC1061cpxA146::Tn10 S. Nakayama (19)
 TB1 ara Δ(lac-proAB) rpsL (φ80lacZΔM15) hsdR T. C. Johnston (11)
Plasmids
 pHW848 virF′-′lacZ fusion gene cloned into pHSG595, a pSC101 replicon vectorH. Watanabe (19)
 pACYC177p15A replicon cloning vectorA. C. Y. Chang (5)
 pSN10182,499-bp BglII-StuI fragment consisting almost entirely of cpxR-cpxA operon cloned intoBamHI-SmaI site of pACYC177This study
 pOK1011,508-bp DraI-StuI fragment consisting almost entirely of cpxA reading frame cloned into expression vector pINIIIA3P. Silverman (24)
 pKH5002Cloning vector for gene disruptionH. Ohmori (20)
 pSN1216K+3-kb EcoRI fragment containing cpxR gene cloned into pKH5002 followed bycpxR reading frame disrupted by insertion of 1.4-kb Kmr cassetteThis study
 pMALTM-c2MalE fusion vectorCommercial product of New England Biolabs
 pMAL-CpxR14 cpxR reading frame fused in frame to malE gene on pMALTM-c2This study
 pHSG397pMB1 replicon cloning vectorT. Hashimoto (30)
 pSN600-T547-bpHpaI-ClaI fragment containing a part ofvirF gene cloned into pHSG397 followed by insertion ofrrnB T1 terminator at the ClaI siteThis study