Table 1.

Primer sequences

PrimerSequenceaPurposeb
ARG5-5DRAAGTGTTTCTTAGAGCAAAACTTGCGTTTGGTGACAGCTTTGGAAAAAATTGGTGTTCACGTTTTCCCAGTCACGACGTTDisruption of ARG5
ARG5-3DRATCTTGGCAAATGTTGTAATAAATCGTGAATTTCCTTGATCTTCAATTTAGTACCATATTTGTGGAATTGTGAGCGGATADisruption of ARG5
ADE2-5DRGTCCATTATATGCTGAAAAATGGTGTCCTTTCACCAAAGAATTGGCTGTGGTTTTCCCAGTCACGACGTTDisruption of ADE2
ADE2-3DRGGGTTGCCTTATCACCCAAGACATTCAACATAATAGCATTGGTGATGGAATGTGGAATTGTGAGCGGATADisruption of ADE2
ARG4-N2KATTCGGATCCGGTACCCCCCTTTAGTAAGATTTTTCAAGAGTAGARG4cloning
ARG4-CSATTCTCGAGCCCGGGCAATGCTTGAGGAGAAGAATCAGAACGCARG4cloning
ca-ura-5TTGGATGGTATAAACGGAAACAURA3cloning
ca-ura-3TCTAGAAGGACCACCTTTGATTGURA3cloning
ca-his-5CCTGGAGGATGAGGAGACAGHIS1 cloning
ca-his-3CCAATATATCGGTTGCACCAHIS1 cloning
5-detectGTTTTCCCAGTCACGACGTTGTAAAACGACDetection of vector sequences flanking disruption marker
3-detectTGTGGAATTGTGAGCGGATAACAATTTCACDetection of vector sequences flanking disruption marker
hisG-NCGCGATACAGACCGGTTCAGACAGGADetection ofhisG sequences
hisG-CTGGTCTTTACTCCATCACAGGGTTCCDetection ofhisG sequences
RIM101-5DRACGATCATTGTGTGACGACCATGTTGGTAGAAAGTCTTCGAACAATTTGTCATTGACTTGTGTGGAATTGTGAGCGGATADisruption of HRM101
RIM101-3DRACATGGACTCTCAAGTGAGAAGTAATGTGATCTCTCTAACTGTAGTTGTGCCACAATTTTTTTCCCAGTCACGACGTTDisruption of HRM101
RIM101-5aGGGGAATTCGTGCTAATCAATCTAACACCACAGCTCTGCDetection of HRM101 alleles
seq7GGTGAACTCAGCCAGAACCTGCGDetection of HRM101alleles
PalA-5DRGCAGCACAAGAGTTAATTAAGAAAGTAGATAAAATGAAACAATATTTGTTACAGGCTAACAACGGAGATGGGTGGAATTGTGAGCGATADisruption of ENX3
PalA-3DRGGAACGAGTTACTAATAGCTAATTCTAAGTCTCGACTCTCAACTCTTCGTCTATACAAATATTCCTCACTTTCCCAGTCACGACGTTDisruption of ENX3
PalA5′ACTGATGATGCAGCACAAGAGDetection ofENX3 alleles
PalA3′CCAGGTTTACTAATAGTCGGDetection of ENX3 alleles
  • a Boldface sequences in 5DR and 3DR primers are segments that anneal to plasmids pGEM-HIS1, pGEM-URA3, and pRS-ARG4ΔSpeI for amplification of disruption cassettes.

  • b Relevant use of primer in this study.