Table 1.

Molecular and kinetic properties of purified PTA from eubacteria and M. thermophila

OrganismMolecular massa(kDa)Apparent Kmb(mM)Reference(s)
Native enzymeSubunitAc-CoAAc-PPiCoA
Thermotoga maritima 170 (α4)340.0230.0240.110.03This work
Methanosarcina thermophila 52 (α)430.170.09 21
Clostridium thermoaceticum 88 (α4)20 11
Clostridium acidiurici 63–75 31
Clostridium kluyveri 38–410.79–1.20.78170.12 3,18
Bacillus subtilis 900.060.48100.096 28
Escherichia coli 160–250 (α2)8130.32 37,46
Lactobacillus fermenti 680.087 26
Rhodopseudomonas palustris 55 (α)52.54.70.15 42
Veillonella alcalescens 75–85 (α2)32–40195 45
  • a Molecular masses of native enzymes were determined by gel filtration, and those of subunits were determined by SDS-PAGE. The proposed subunit composition of the native enzyme is given in parentheses.

  • b Ac, acetyl; P, phosphate; Pi, inorganic phosphate; —, not determined.