Table 3.

Relative heat shock mRNA levelsa

StrainLevel of the following specific mRNAb:
hrcAdnaKdnaJhflB
BK8 (dnaK+-erm)1.01.01.01.0
BK11 (dnaKΔ2)3.03.21.21.1
BK6 (dnaKΔ1)147.32.31.4
  • a The strains were grown in GM17 supplemented with erythromycin at 30°C.

  • b Northern blotting was performed with 10 μg of total RNA from each strain. Specific mRNA species were detected with radioactive double-stranded DNA probes covering the gene of interest. Radioactivity was measured in each lane with a Packard Instant Imager.