Table 2.

Plasmids

PlasmidRelevant featuresaSource or reference
pAK80Reporter geneslacLM (L. lactis, E. coli) 15
pCI3340Shuttle vector (L. lactis, E. coli) 10
pGh9:ISS1 Orit s, used for ISS1mutagenesis (L. lactis, E. coli) 21
pJM1000 HindIII rescue plasmid from MK136 containing the purR promoter (E. coli)This work
pJM1010Internal purR fragment in pRC1 (E. coli)This work
pMK1000 EcoRI rescue plasmid from MK136 containing the purR gene (E. coli)This work
pMK1030 purR gene and promoter inserted into the XhoI and HindIII sites of pCI3340 (L. lactis, E. coli)This work
pMK1033 purR promoter fused to thelacLM genes in pAK80 (L. lactis, E. coli)This work
pRC1Ermr (E. coli) 19
  • a The plasmid can replicate in the genera shown in parentheses.