Table 1.

E. coli K-12 strains and plasmids used in this study

Strain or plasmidaGenotype or propertyReference
KS272FΔlacX74 galE galK thi rpsL ΔphoA16
SF110KS272 degP ompT16
SF115KS272 degP ptr16
SF120KS272 depP ptr ompT16
UT5600Fara-14 leuB6 azi-6 lacY1 proC14 tsx67Δ(ompT-fepC266) entA403 trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-14
pDS319Tetr; carries an ompT containing 2-kb E. coli chromosomalEcoRI-PstI fragment ligated with the largeEcoRI-PstI fragment from plasmid pBR322
pML19Ampr; carries an ompT containing 2-kb E. coli chromosomal EcoRI-PstI fragment ligated with the large EcoRI-PstI fragment from plasmid pUC197, 8
  • a The source for all strains and plasmids was G. Georgiou.