Table 1.

E. coli K-12 strains and plasmids used in this study

Strain or plasmidaGenotype or propertyReference
KS272FΔlacX74 galE galK thi rpsL ΔphoA16
KS474KS272degP16
SF103KS272ptr16
SF110KS272 degP ompT16
SF115KS272 degP ptr16
SF120KS272 depP ptr ompT16
UT5600Fara-14 leuB6 azi-6 lacY1 proC14 tsx67Δ(ompT-fepC266) entA403 trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-14
pDS319Tetr; carries an ompT containing 2-kb E. coli chromosomalEcoRI-PstI fragment ligated with the largeEcoRI-PstI fragment from plasmid pBR322
pML19Ampr; carries an ompT containing 2-kb E. coli chromosomal EcoRI-PstI fragment ligated with the large EcoRI-PstI fragment from plasmid pUC197, 8
  • a The source for all strains and plasmids was G. Georgiou.