Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant phenotype of characteristic(s)Source or reference
Strains
P. aeruginosa
  PAO1Prototrophic13
  PAO-JP1ΔlasI::tet, LasI null mutant27
  PAO-R1SmrΔlasR::tet, LasR null mutant9
  PDO111 rhlR::Tn501derivative of PAO1, Hgr, RhlR null mutant4
E. coli
  MG4 λI14Δ(argF-lac)U169 zah-735::Tn10 recA56 srl::Tn10 lasI::lacZ lysogen35
  MG4 λB2 Δ(argF-lac)U169 zah-735::Tn10 recA56 srl::Tn10 lasB::lacZ lysogen35
Plasmids
 pEX1.8Ampr, stabilizing fragment (1.8-kb PstI fragment)27
 pEXRAmpr lasR pwt This study
 pEXRLAmpr rsaL ptac This study
 pEXRRAmpr rsaL ptac (800-bpSalI-HphI fragment)lasR pwt This study
 pEXRR2Ampr rsaL ptac (307-bpSfiI-HphI fragment)lasR pwt This study
 pEXRR/NSAmpr rsaL ptac (start methionine ATG to CTG) lasR pwt This study
 pPCS2001Ampr lasI-lacZ This study
 pPCS2002Ampr rsaL-lacZ This study
 pKDT17Ampr lasB-lacZ lasR plac 25
 pPCS15Ampr lasB-lacZ lasR plac rsaL pwt This study
 pTS4001.7Ampr lasB-lacZ lasR pwt rsaL pwt 23
 pPCS16Ampr lasB-lacZ rsaL plac lasR pwt This study
 pPCS15/rsaL-lacZ Ampr lasR plac rsaL pwt-lacZ This study
 pTS4001.7/rsaL-lacZ Ampr lasR pwt rsaL pwt-lacZ This study
 pPCS16/rsaL-lacZ Ampr lasR pwt rsaL plac -lacZ This study
 pPCS16/ESAmpr lasB-lacZ rsaL plac (codon 3 TCA to TAA)lasR pwt This study
 pPCS16/NSAmpr lasB-lacZ rsaL plac (start methionine ATG to CTG)lasR pwt This study
 pTS400Ampr, lasB-lacZ translational fusion on pSW20523
 pSW205Ampr lacZ′, stabilizing fragment (1.8-kb PstI fragment)10
 pSWRLAmpr rsaL-lacZ(out-of-frame translational fusion)This study
 pSWRL2Ampr rsaL-lacZ (in-frame translational fusion)This study
 pTrcHisAmpr, A, B and C vectors for cloning in all three framesInvitrogen