Table 1.

Growth conditions for culturesa

Growth conditionBuffer (100 mM)OxygenpHbGrowth time (h)
AcidHOMOPIPES or MES+4.413–20
4.540–50
NeutralPIPES or MOPS+7.0 1.7
7.0 3  
BaseAMPSO or TAPS+9.210–35
9.024–48
Propionic acidMES with 50 mM propionate +6.053  
6.224  
  • a All media contained buffered LBK. Cultures were grown to an optical density at 600 nm of 0.35 to 0.50 at 37°C, with or without aeration as indicated.

  • b Initial pH of broth at inoculation. Final pH after growth changed by less than 0.3 U.