Table 3.

Effects of bkdR and sigL mutations on ptb′-′lacZ expression

StrainRelevant genotypeβ-Galactosidase activity (Miller units/mg of protein) in the following minimal mediuma:
MMMM + isoleucine (20 mM)MM + α-keto-β-methylvalerate (20 mM)MM + valine (20 mM)MM + α-keto-isovalerate (20 mM)
QB7501 amyE::ptb′-′lacZ 552,2659501,145400
QB7502 amyE::ptb′-′lacZ sigL::aphA3 55ND5ND
QB7511 amyE::ptb′-′lacZ bkdR::aphA3 51ND5ND
QB7530 amyE::ptb′-′lacZ bkdR::aphA3pBkdR11402,440ND2,120ND
  • a ND, not determined.