Table 1.

B. subtilis strains used in this study

StrainGenotype or descriptionaSource or referenceb
168 trpC2 1
QB5505 trpC2 sigL::aphA3 5
QB7501 trpC2 amyE::ptb′-′lacZ (ΔA)This work
QB7502 trpC2 amyE::ptb′-′lacZ sigL::aphA3 QB5505 DNA→QB7501
QB7505 trpC2 amyE::ptb′-′lacZ tnrA::erm SF706T DNA→QB7501
QB7507 trpC2 lpd::pMutin4This work
QB7511 trpC2 amyE::ptb′-′lacZ bkdR::aphA3 pBkdR2 lin→QB7501
QB7512 trpC2 bkdR::aphA3 pBkdR2 lin→168
QB7513 trpC2 lpd::pMutin4bkdR::aphA3 pBkdR2 lin→QB7507
QB7514 trpC2 bcd::pMutin4This work
QB7515 trpC2 bcd::pMutin4bkdR::aphA3 pBkdR2 lin→QB7514
QB7517 trpC2 amyE::ptb′-′lacZ (ΔB)This work
QB7518 trpC2 amyE::ptb′-′lacZ (ΔC)This work
QB7519 trpC2 amyE::ptb′-′lacZ (ΔD)This work
QB7520 trpC2 amyE::ptb′-′lacZ (ΔE)This work
QB7521 trpC2 bkdB::pMutin4This work
QB7522 trpC2 amyE::ptb′-′lacZ codY::erm PS258 DNA→QB7501
QB7530 trpC2 amyE::ptb′-′lacZ bkdR::aphA3 pBkdR1This work
PS258 trpC2 codY::erm A. L. Sonenshein
SF706T trpC2 tnrA::Tn917 amyE::gabP-lacZ 38
  • a ΔA, ΔB, ΔC, ΔD, and ΔE are defined in Fig. 5.

  • b lin, linearized. pBkdR1 and pBkdR2 were constructed as discussed in Materials and Methods.