Table 4.

Induction of the expression of bcd,lpd, and bkdB genes

StrainRelevant genotypeβ-Galactosidase activity (Miller units/mg of protein) in the following minimal mediuma:
MM + glucoseMM + glucose + isoleucine (20 mM)
Without IPTGWith IPTGWithout IPTGWith IPTG
QB7514 bcd::pMutin4355101,1701,100
QB7515 bcd::pMutin4bkdR::aphA3 2232
QB7507 lpd::pMutin43202802,8002,630
QB7513 lpd::pMutin4bkdR::aphA3 5534
QB7521 bkdB::pMutin4290ND1,150ND
  • a ND, not determined.