Table 1.

Bacterial strains and plasmids used

Strain or plasmidGenotype or descriptionReference(s) or source
Escherichia coli
 2787 (O126:H27)AIDA-I+ 6
 BD1302 metB Δ(ppc-argECBH oxyR)relA spoT+) Hfr P4X 25
 BR83 topA57 argA supD74 rpsL 50
 C600F thr-1 leuB6 lacY1 fhuA21 supE44 thi-1 mcrA 4
 C600 Δhns C600 Δhns 69
 CS1291F ompC::Tn5 rpsL gyrA 68
 CSH26 araΔ(lac-proP) thi 76
 CSH26 Δcya CSH26 Δcya 76
 CSH50 ara Δpro-lac thi 41
 CSH50fis CSH50fis::kan 41
 DL379F araΔ139Δ(lacIPOZYA-argF)U169 rpsL thi-1 λ246 lysogen (papBAp-lacZYA) 9
 DL850DL379mbf-20::mTn10 16
 GM2929F ara-14 leuB6 lacY1 tsx-78 supE44 galK2 galT22 hisG4 rfbD rspL136 xyl-5 mtl-1 thi-1 glnV44 fhuA31 dam-13::Tn9(Cmr) dcm-6 hsdR2 recF143 mcrA mcrB 10, 59
 GM3819F thr-1 ara-14 leuB6Δ(gpt-proA)62 lacY1 tsx-33 supE44 galK2 hisG4(Oc) rfb-1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 dam-16::Kanr 60
 GSO5SA2692 ΔoxyR::kanY2055 oxyS-galK) 43
 GSO9SA2692 ΔoxyR::kan G. Storz
 HB101 hsdS20 recA13 proA2 leu-6 thi-1 rpsL20 ara-14 galK2 lacY1 xyl-5 mtl-1 supE44 merB endA 15
 IRH10ML308-225dsbA by P1cml from JCB571This study
 IRH11ML308-225 degP by P1cml from KS474This study
 IRH12ML308-225 dam-16 by P1cml from GM3819This study
 IRH13ML308-225dam-13::Tn9 by P1cml from GM2929This study
 IRH14ML308-225 ΔoxyR::kanY2055 oxyS-galK) by P1cml from GS05This study
 IRH15ML308-225 ΔoxyR::kanby P1cml from GS09This study
 IRH16IRH14 containing pAQ25This study
 IRH17IRH14 containing pGSO68This study
 IRH18IRH14 containing pGSO69This study
 IRH19BD1302 dam-16 by P1cml from GM3819This study
 JCB571 araD139Δ(araABC-leu)7679 galU galKΔ(lac)X74 rpsL thi phoR zih-12::Tn10 dsbA::Kan-1 5
 KNS4MC4100 Φ(hns-lacZ) hyb-2(Kanr) 24
 KS474F ΔlacX74 galE galK thi rpsL(strA) ΔphoA (PvuII)degP41PstI-Kanr) 72
 M182Δ(lacIPOZY)X74 galK galU strA 21
 M182 Δcrp M182 Δcrp 17
 MC4100F araΔ139Δ(argF-lac)U169 deoC1 flb-5301 relA1 rpsL150 ptsF25 rbsR 20
 ML308-225 (O13:O68:H) lacIZ lacY+lacA+ Ag43+ 57
 OHP250C600hupB::Kanr 34
 OHP251C600hupA::Cmr 34
 RM269 strR galK2 Δ(srl recA)srl::Tn10 [pGBK1 Φ(gyrB galK)] 51
 SA2692HB101 recA+ Δlac Δgal-165 43
 UT5600F ara-14 leuB6 azi-6 lacYI tsx-67 proC trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-1 entA403Δ(ompT-fecC266) ΔompP 40
 W3110Fλ 4
 ZK126W3110 ΔlacU169 tna-2 rpoS + 23
 ZK1000ZK126rpoS::kan 11
Edwardsiella tarda ATCC 15947Type strain
Enterobacter cloacae ATCC 13047Type strain
Erwinia carotovora NCPPB 312Type strain
Klebsiella pneumoniae ATCC 13883Type strain
Proteus mirabilis ATCC 13315Type strain
Salmonella typhimurium ATCC 14028Type strain
Serratia marcescens ATCC 13880Type strain
Shigella flexneri type 26 ATCC 12022Type strain
Plasmids
 pBluescript II SK(+)ColE1oriV lacZα Ampr phagemid cloning vectorStratagene
 pAQ25 oxyR wild type in pKK177-3 with altered Shine-Dalgarno sequence to allow overproduction of OxyR; Ampr 71
 pGSO68 oxyR C199S in pKK177-3; Ampr 43
 pGSO69 oxyR A233V in pKK177-3; Ampr 43