Table 1.

Plasmids and mutants of Synechocystis used and constructed in this study (see also Fig. 2)

DesignationSize (kb)Description
pGGM024.32pGEM7 containing a 1.3-kb BamHI/EcoRI fragment, the coding sequence of slr0214 amplified by PCR
pUC19GM4.0pUC19 containing a 1.3-kbBamHI/EcoRI fragment, the coding sequence ofslr0214 amplified by PCR
pGGMK29−5.6pGGM02 containing inactivated slr0214 (aphII gene inserted at the HincII site opposite to the direction of transcription of slr0214)
pGGML056.3pGEM7 containing a 3.3-kb BamHI/PstI fragment, the coding sequence of slr0214 with an additional 1 kb upstream and 1 kb downstream amplified by PCR
pGGMDE12−6.3pGGM02 containing partially deleted slr0214 (aphII gene inserted, after deletion of the internal BalI fragment, opposite to the direction of transcription of slr0214)
pGEXGM6.2pGEX4T3 containing a 1.3-kbBamHI/EcoRI fragment, the coding sequence ofslr0214 fused to GST
slr0214 mutantSynechocystis mutant obtained after transformation of the WT with pGGMK29−
slr0214Δ mutantSynechocystis mutant obtained after transformation of the WT with pGGMDE12−