Table 2.

Cholesterol uptake in upc2-1 andupc2::URA3 strains

StrainRelevant genotypeaMean cholesterol uptake (μg of cholesterol/mg [dry weight] of cells) ± SD with plasmidb:
YpH-1pUPC2
463-1D UPC2 0.232 ± 0.0410.214 ± 0.044
SC2-1C upc2-1 4.68 ± 1.10.771 ± 0.059
CJ625 upc2::URA3 0.160 ± 0.0180.178 ± 0.024
CJ296 UPC2/UPC2 0.174 ± 0.0180.178 ± 0.024
CJ571 upc2-1/UPC2 1.01 ± 0.0650.226 ± 0.037
CJ573 upc2-1/upc2::URA3 3.82 ± 0.270.601 ± 0.21
CJ577 upc2-1/upc2-1 2.56 ± 0.790.573 ± 0.019
CJ581 upc2::URA3/upc2::URA3 0.279 ± 0.0220.470 ± 0.33
  • a In this tableydr213::URA3 is designatedupc2::URA3.

  • b Plasmid YpH-1 is the control plasmid lacking the YDR213W open reading frame, and plasmid pUPC2 contains the entire YDR213W coding sequence with the native 5′ untranslated sequence.