Table 3.

Transposition of Tn5520in E. coli

Donor Transposition frequency (mean ± SE)a
DW1030(pOX38::Kan, pTJ20)b (1.1 ± 0.2) × 10−5
DW1030(pOX38::Kan, pBR322)c (7.7 ± 1.9) × 10−8
pTJ22.23d (1.9 ± 1.2) × 10−7
pTJ22.26d (1.2 ± 0.05) × 10−7
pTJ22.20d (1.2 ± 0.3) × 10−7
pTJ22.25e (7.5 ± 2.3) × 10−5
pTJ22.10e (2.9 ± 0.9) × 10−5
pTJ22.36e (1.9 ± 0.6) × 10−5
pTJ22.24e (5.1 ± 2.9) × 10−5
pTJ23f (7.7 ± 1.9) × 10−8
  • a Experiments were performed at least in triplicate.

  • b Positive control.

  • c Negative control.

  • d Mutant with Tn1000 insertion inbipH.

  • e Mutant with Tn1000 insertion outside bipH.

  • f Deletion of Tn5520 B end.