Table 1.

Bacterial strains and plasmids used

Strain or plasmidRelevant characteristicsSource or reference
Strains
S. meliloti
  Rm5000 S. meliloti SU47, rif-5 9
  MK5001Same as Rm5000 but withkatA::Tn5 18
  MK5002Same as Rm5000 but withkatC::ΩSmr/Spr This work
  MK5003Same as MK5002 but withkatA::Tn5 This work
  RKCZ01Same as Rm5000 but with pkatC-lacZ fusionThis work
E. coli
  DH5αF supE44 ΔlacU16980dlacZΔM15)hsdR17 (rK mK +) recA1 endA1 gyrA96 thi-1 relA1 Bethesda Research Laboratories
  MT607 pro-82 thi-1 hsdR17 supE44 recA56 10
  MT616MT607(pRK600) 10
Plasmids
 pLAFR1IncP cosmid cloning vector, Tcr 12
 pRK415IncP cosmid cloning vector, Tcr 21
 pRK600ColE1 replicon with RK2 transfer region, Cmr 10
 pHP45ΩAmpr pBR322 derivative with ΩSmr/Spr 8
 R751-pGM2IncP GmrSmr 19
 pBluescriptKS(+)Derivative of pUC19 with f1(+),oriR Ampr Stratagene
 pSUP202ColE1, Mob+ Tcr AmprCmr 31
 pKOK5Ampr Kmr, pSUP202 derivative; source of lacZ-Kmrcartridge 22
 pLRK2pLAFR1, S. meliloti cosmid clone with katC This work
 pBSKC1pBluescript, 3.3-kb EcoRI-PstI fragment with partial katC This work
 pBSKC2pBluescript, 1.5-kb SacI-ApaI fragment with partial katC This work
 pBSKC3pBluescript, 2,190-bp EcoRI-ApaI fragment with partial katC This work
 pSUPKCpSUP202, PstI-PstI fragment of pBSKC3This work