Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference or source
E. coli strains
 HB101supE44 hsdS20 recA13 ara-14 proA2 lacY galK2 rpsL20 xyl-5 mtl-12
 JM109recA1 supE44 endA1 hsdR17 gyrA96 relA1 thiΔ(lac-proAB)33
 CLEO1HB101 transformed with pMC1; TcrCmrThis work
S. mutansstrains
 UA130Serotype c; IPS+P. Caufield
 GMS100UA130 with dlt::catfusion from pMC1; TcrThis work
 BM71PTS+3, 16
 DC10ptsI::ermPTS8
Plasmids
 pAM620pVA891::pAD1EcoRIF′::Tn916Tcr Emr32
 pMH109Contains promoterless cat gene preceded by pUC13 MCS; Tcr12
 pNC10pMH109 derived; contains promoterless catgene; Tcr ColE1This work
 pNC12pNC10 derived; ColE1 tetMThis work
 pMC1pNC12 derived; ColE1dlt::cat tetM CmrThis work
 pDLT1-23.1-kb S. mutans amplicon containing dlt1 and dlt2 in pGEM-T Easy; AprThis work
 pDLT30.3-kb S. mutans amplicon containing dlt3 in pGEM-T Easy; AprThis work
 pDLT3-41.7-kb S. mutans amplicon containing dlt3 and dlt4 in pGEM-T Easy; AprThis work
 pYA30236.9-kbBglII fragment containing S. mutans dlt in pUC19; Apr28
 pYA30318-kbXbaI fragment containing S. mutans dlt in pUC19; AprThis laboratory