Table 2.

Transfer of Tn5520 in B. fragilis and mobilization in E. coli

ProcessDonor strainPlasmidFrequency of transfer (mean ± SE)a
Plasmid transfer (B. fragilis to E. coli)TM4000pGAT400(7.9 ± 3.8) × 10−9
pGAT400ΔBglIINDb
pHB23Δ5(4.5 ± 3.1) × 10−8
TM4.23pGAT400(3.6 ± 1.6) × 10−5
pGAT400ΔBglIIND
pHB23Δ5(1.5 ± 0.83) × 10−4
R751 mobiliza-tion (E. coli toE. coli)HB101(R751)c pBR328ND
pGAT400(0.4 ± 0.17) × 10−2
pTJ20(3.2 ± 0.05) × 10−1
pKB1(0.3 ± 0.1) × 10−2
pGV2d ND
pGV3d ND
pGV4e (3.7 ± 2.9) × 10−7
pGV5e ND
pGV6e (3.3 ± 1.5) × 10−6
pGV7f (1.6 ± 0.09) × 10−2
pGV8f (1.02 ± 0.095) × 10−2
pGV9f (1.4 ± 0.05) × 10−2
  • a Experiments were performed at least in triplicate.

  • b ND, transfer or mobilization not detectable (≤10−9 [Bacteroides] or no transconjugants on selective plates).

  • c The IncPβ plasmid R751 was coresident in the donor during E. coli-to-E. colimobilization experiments.

  • d Tn1000 insertion inbmpH.

  • e Tn1000 insertion proximal tobmpH.

  • f Tn1000 insertion distal tobmpH.