Table 2.

PCR primers used in this study

PrimerNucleotide sequencePosition (5′ end)a
RR3015′ GAG AAC GGA TTC AAC GCC ACC 3′348 bp left of INT1 in strain F1
RR3025′ ATC GGC AAA CTG TGC CAT GAC TGG 3′384 bp right of INT2 in strain F1
RR3035′ ACT GCA GCA GAG CAC GCC GTT CG 3′523 bp right of INT1 in strain F1
RR3155′ TGC TCT CAG TTC CCG CAT CC 3′ clc element, 209 bp from left end, directed inward
RR3165′ GAT GAC GTT GTG ACG ACT GC 3′ clcelement, 178 bp from left end, directed outward
RR3255′ AGA TAC TGC GCA GCG ACA ACA CCA 3′295 bp left of INT2 in strain F1
  • a Relative to the positions of the exact junctions between the clc element and chromosomal target sites in strain F1.