Table 1.

Plasmids used in this study

PlasmidSource of insert DNARelevant characteristics
pDC1004.2-kbBglII fragment of pAC27 (9)Contains theclcABD genes
pRR1014.2-kbNheI-HindIII fragment of cosmid insert 3C9 cloned into pUC18NotContains the right junction (R1) of the integrated clc element at INT1
pRR1021.9-kbNotI-HindIII fragment of pRR101 cloned into pUC28Contains the right junction (R1) of the integratedclc element at INT1
pRR1044.2-kbEcoRI fragment of cosmid insert 3G3 cloned into pUC18NotContains the left junction (L1) of the integratedclc element at INT1
pRR1084.1-kbNheI-EcoRI fragment of cosmid insert 2B1 cloned into pUC18NotContains the right junction (R2) of the integratedclc element at INT2
pRR123871-bp PCR product obtained from strain F1 DNA with primers RR301 and RR303, cloned into pGEM-T EasyContains INT1 in strain F1 prior to integration
pRR148984-bp iPCR product obtained from strain RR221 DNA with primers RR315 and RR316 after digestion with SphI and religation, cloned into pGEM-T EasyContains the left junction (L2) of the integrated clc element at INT2
pRR1571.1-kb PCR product obtained from strain F1 DNA with primers RR302 and RR325, cloned into pGEM-T EasyContains INT2 in strain F1 prior to integration