Table 2.

Effect of phhB or DCoH supplied intrans upon expression of phhA in transcriptional or translational fusionsa

Plasmid used in transGene supplied intransβ-Galactosidase activityb
pJS51ZλJS1pJS105Z
pJZ9-4 phhB 180.7 ± 40.613.2 ± 1.213.1 ± 0.1c
pGST-DCoH DCoH 190.6 ± 13.114.1 ± 0.810.4 ± 0.3c
pUC18None182.3 ± 16.315.7 ± 1.27.3 ± 0.7
  • a Regulation of phhAexpression was studied with lacZ as the reporter gene. β-Galactosidase activities in the transcriptional fusions pJS51Z (phhA′-lacZ) (multicopy) and λJS1 (phhA′-lacZ) (single copy) and the translational fusion pJS105Z (phhA′-′lacZ) (multicopy) were assayed in the absence and presence of either PhhB or DCoH.

  • b β-Galactosidase activities are reported in Miller units. Values are means (± standard deviations) of results from three independent experiments.

  • c Significantly higher than the value for the control (pUC18) (P < 0.0001 by analysis of variance).