Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
E. coli
 JM109recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+ lacIqlacZΔM15]Takara
 1321pckA maeA maeB14
 SM10 λpirthi-1 thr leu tonA lacY supE recA::RP4-2Tc::Mu, Kmr λpir27
 P2392e14 (McrA) hsdR514 supE44 supF58 lacY1 or Δ(lacIZY)6 galK2 galT22 metB1 trpR55 (P2 lysogen)Stratagene
R. palustris
 No. 7Wild type10
ppcmutantppc::Kmr18
pckAmutantpckA::KmrThis work
pckA ppcmutantpckA::Tcrppc::KmrThis work
Plasmids
 pUC118Apr; α-lac/MCS M13oriTakara
 pGEM-TApr; α-lac/MCS; PCR cloning vectorPromega
 pMC1871Tcr;lacZ fusion vectorPharmacia
 pUC4KApr Kmr; source of Kmr cartridgePharmacia
 pGP704Apr; oriR6K, mobRP427
 pMG102Kmr; pHSG298 derivative; E. coli-Rhodopseudomonas sp. shuttle vector17
 pMG200Apr; pUC118 with a 2.5-kbSmaI-PstI fragment containing the R. palustris No. 7 pckA geneThis work
 pMG201Kmr; pMG102 with a 2.5-kb XbaI PCR-amplified fragment containing the R. palustris No. 7pckA geneThis work
 pMG202Kmr; pMG102 with a 5.0-kb SalI fragment containing thepckA-lacZ fusionThis work
 pMG300Apr Gmr; 1.75-kbEcoRI fragment of Gmr cartridge inserted into pGP704 EcoRI site18
 pMG312AprKmr Gmr; 2.8-kb KpnIpckA::Kmr fragment cloned into pMG300KpnI siteThis work