Table 2.

Plasmids used

NameDescriptionReference
pRC1Emr, 3.5-kb cloning vector constructed from pBluescript KSII (+) 29
pCL52Emr, 2.4-kb cloning vector constructed from pBluescript KSII (+)Unpublished data
pCL1025.5-kb HindIII fragment from strain NCDO763 in pRC1This work
pCL1323.2-kbHindIII fragment from strain NCDO763 in pCL52This work
pCL133Same as pCL132 but inserted in opposite orientationThis work
pCL1349-kb EcoRI fragment from strain NCDO763 in pCL52This work
pCL1361.7-kbEcoRV fragment from pCL134 in pCL52This work
pCL137Same as pCL136 but inserted in opposite orientationThis work
pCL1381.4-kb PCR product from strain NCDO763 in pCL52This work
pCL139Same as pCL138 but inserted in opposite orientationThis work
pCL2005.4-kbSacI fragment from strain MG1363 in pCL52This work
pCL2019-kb AccI fragment from strain MG1363 in pCL52This work
pCL2031.7-kb EcoRV fragment from pCL200 in pCL52This work
pCL204Same as pCL203 but inserted in opposite orientationThis work
pCL2061.5-kbBstXI fragment from pCL201 in pCL52This work
pCL207Same as pCL206 but inserted in opposite orientationThis work
pCL2081.5-kbAflIII-BstXI fragment from pCL201 in pCL52This work
pCL209Same as pCL206 but inserted in opposite orientationThis work
pCL2101-kbEcoRV-SacI fragment from pCL200 in pCL52This work
pCL2111-kb EcoRV fragment from pCL200 in pCL52This work
pCL212Same as pCL211 but inserted in opposite orientationThis work