Table 1.

Strains and plasmids included in this study

K. pneumoniae strain or plasmidRelevant phenotypeSource or reference
Porin-sufficient strainsa
 SD8OmpK36+OmpK35+OmpK37 13
 CSUB10SOmpK36+ OmpK35OmpK37 4
Porin-deficient strainsb
 CSUB10ROmpK36OmpK35 OmpK37 4
 LB4OmpK36 OmpK35OmpK37+ 18
 LB66OmpK36 OmpK35OmpK37+ 15
Plasmids
 pBluescript KS(−)High-copy-number cloning vector, Apr Stratagene
 pGEM-TPCR fragment cloning vector, Apr Invitrogen
 pWSK29Low-copy-number cloning vector, Apr 29
 pCSI2Kmrcassette-containing vector 9
 pSUV7pACYC184 with clonedompK36 3
 pSHA25KpWSK29 with cloned ompK36 gene and Kmr cassette from pCSI2This work
 pSHA16pWSK29 with clonedompK35 Our laboratory
 pSHA16KpWSK29 with clonedompK35 gene and Kmr cassette from pCSI2This work
 pQE1pWSK29 carrying ompK37-containingEcoRI fragmentThis work
 pQE1KpQE1 with cloned Kmr cassette from pCSI2This work
 pQE3pBluescript KS(−) carrying ompK37-containingBamHI fragment from pQE1This work
 pQE3KpQE3 with cloned Kmr cassette from pCSI2This work
 pQE7pWSK29 carrying ompK37 transcribed bylac promoterThis work
 pQE7KpQE7 with cloned Kmr cassette from pCSI2This work
 pQE31pBluescript KS(−) containing ompK37 and 700 bp of ompK37 upstream flanking sequenceThis work
 pQE31KpQE31 with cloned Kmr cassette from pCSI2
 pQE33pBluescript KS(−) containing ompK37 and 200 bp of ompK37 upstream flanking sequenceThis work
 pQE33KpQE33 with cloned Kmr cassette from pCSI2This work
  • a Porin-sufficient strains are strains expressing either porin OmpK36 or OmpK35 or both.

  • b Porin-deficient strain are strains lacking expression of porins OmpK36 and OmpK35.