Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeaReference or source
Strains
E. coliDH10BFφ80dlacZΔM15 recA1 Gibco/BRL, Gaithersburg, Md.
S. carnosusTM300 13
S. aureus
  RN4220NCTC 8325-4-r (restriction mutant) 19
  8325NCTC 8325
  COLHigh-Mcr clinical isolate
  NewmanHigh level of clumping factor 9
  8325 ΔsigB Deletion of sigB operon, Emr This study
  COL ΔsigB Deletion of sigB operon, Emr This study
  Newman ΔsigB Deletion of sigB operon, Emr This study
Plasmids
 pBLSK(+)Apr Stratagene, La Jolla, Calif.
 pIKET tetK and ermB in pBLSK+; Apr in E. coli; Tcr Emrin S. aureus This study
 pTX15TcrPXyl; staphylococcal origin of replication 28
 pIK581.1-kbNsiI and 1.7-kb HindIII S. aureusfragments in pIKETThis study
 pIK64800-bp sigBfragment in pTX15This study
  • a Abbreviations are as follows: Apr, ampicillin resistant; Emr, erythromycin resistant; Mcr, methicillin resistant; Tcr, tetracyclin resistant.