Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeaReference or source
Strains
E. coliDH10BFφ80dlacZΔM15 recA1Gibco/BRL, Gaithersburg, Md.
S. carnosusTM30013
S. aureus
  RN4220NCTC 8325-4-r (restriction mutant)19
  8325NCTC 8325
  COLHigh-Mcr clinical isolate
  NewmanHigh level of clumping factor9
  8325 ΔsigBDeletion of sigB operon, EmrThis study
  COL ΔsigBDeletion of sigB operon, EmrThis study
  Newman ΔsigBDeletion of sigB operon, EmrThis study
Plasmids
 pBLSK(+)AprStratagene, La Jolla, Calif.
 pIKETtetK and ermB in pBLSK+; Apr in E. coli; Tcr Emrin S. aureusThis study
 pTX15TcrPXyl; staphylococcal origin of replication28
 pIK581.1-kbNsiI and 1.7-kb HindIII S. aureusfragments in pIKETThis study
 pIK64800-bp sigBfragment in pTX15This study
  • a Abbreviations are as follows: Apr, ampicillin resistant; Emr, erythromycin resistant; Mcr, methicillin resistant; Tcr, tetracyclin resistant.