Table 2.

β-Galactosidase and RubisCO activitiesa in R. sphaeroides CAC and 1312, carrying various fusion plasmid constructs

Strain and plasmidValue for growth condition:
Chemoheterotrophy (malate-air)Photoheterotrophy (malate-argon)Photoautotrophy (1.5% CO2–H2)Chemoautotrophy (5% CO2–45% H2–50% air)
CAC
 pVKB13.9 (2.1)32 (46)467 (392)273 (82)
 pVKC192 (1.3)1,079 (54)7,640 (332)12,635 (66)
 pVKC1ΔXho25 (1.1)295 (48)3,192 (183)3,330 (73)
 pVK14030.0 (1.5)3.0 (66)3.4 (338)0.0 (110)
1312
 pVKH10.7 (1.8)2.5 (5.8)No growth
 pVKB11.7 (2.1)2.7 (8.1)No growth
 pVK14030.0 (2.0)0.2 (8.9)No growth
  • a All enzyme activities are expressed in nanomoles per minute per milligram of protein. All values represent the averages of two to three independent determinations of multiple assays. Values in parentheses represent RubisCO activities in nanomoles of CO2 fixed per minute per milligram of protein.