Table 1.

Characteristics of the strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource or reference
Strains
E. coli
  MC1061F lac 7
  M15F lac Qiagen
  TG1 lacI q 31
V. cholerae
  TRH7000El Tor, Δ(ctxAB), wild type with respect to extracellular protein secretion 20
  Mut8TRH7000 with a Kmr insertion in the epsLgene 45
  PU3TRH7000 with a Tn5-transposon in the epsM gene 37
Plasmids
 pMMB67Broad-host-range plasmid, Apr 15
 pMMB587 epsM (His)6 in pQE70This study
 pMMB606 epsM (His)6 in pMMB67EH repliconThis study
 pMS44 epsL in pMMB67HE 46
 pMS45 (His)6 epsL in pQE30This study
 pMS46 (His)6 epsL in pMMB67EHThis study
 pMS47 epsL (His)6 in pQE60This study
 pMS48 epsL (His)6 in pMMB67EHThis study
 pMS58 epsL epsM in pMMB67HEThis study
 pREP4 lacI q, Kmr Qiagen
 pQE30Codons for six N-terminal histidine residues, Apr Qiagen
 pQE60Codons for six C-terminal histidine residues, Apr Qiagen
 pQE70Codons for six C-terminal histidine residues, Apr Qiagen
 pWD615 etxB (E. coli heat-labile enterotoxin B subunit gene), Tcr 11