Table 1.

Strains and plasmids

Plasmid or strainRelevant characteristicsReference or source
pK18Knr; derivative of pUC18 32
pKC1-5Knr; pK18 containing the 719-bpEcoRI-AvaII fragment of pUC12EHThis study
pKC1ΔXhoKnr; pKC1-5 containing a 4-bp deletion at the XhoI site within the insertThis study
p12EHApr; pUC9 carrying a 1.8-kbpEcoRI-HindIII fragment containingcbbR 16
pMC1403Apr; LacZ translational fusion vector 34
pMC1H1Apr; pMC1403 carrying the 187-bp BssHII-BamHI fragment of pKC1-5This study
pMCB1Apr; pMC1403 carrying the 363-bp XhoI-BamHI fragment of pKC1-5This study
pMCG1Apr; pMC1403 carrying the 413-bpPflMI-BamHI fragment of pKC1-5This study
pMCG1ΔXhoApr; pMCG1 carrying a 4-bp deletion at the XhoI siteThis study
pMCF1Apr; pMC1403 carrying the 584-bp DraIII-BamHI fragment of pKC1-5This study
pMCE1Apr; pMC1403 carrying the 674-bp NarI-BamHI fragment of pKC1-5This study
pMCC1Apr; pMC1403 carrying the 719-bpEcoRI-BamHI fragment of pKC1-5This study
pMCC1ΔXhoApr; pMCC1 carrying a 4-bp deletion at the XhoI siteThis study
pMCD1Apr; pMC1403 carrying the 1,323-bp HindIII-AvaII fragment of p12EHThis study
pVK101KnrTcr; broad-host-range vector 23
pVK1403Apr; pVK101 containing pMC1403 inserted in the EcoRI siteThis study
pVKH1KnrTcr Apr; pVK101 carrying pMCH1 inserted in theEcoRI siteThis study
pVKB1KnrTcr Apr; pVK101 carrying pMCB1 inserted in theEcoRI siteThis study
pVKG1KnrTcr Apr; pVK101 carrying pMCG1 inserted in theEcoRI siteThis study
pVKG1ΔXhoKnrTcr Apr; pVK101 carrying pMCG1ΔXho inserted in the EcoRI siteThis study
pVKF1KnrTcr Apr; pVK101 carrying pMCF1 inserted in theEcoRI siteThis study
pVKE1KnrTcr Apr; pVK101 carrying pMCE1 inserted in theEcoRI siteThis study
pVKC1KnrTcr Apr; pVK101 carrying pMCC1 inserted in theEcoRI siteThis study
pVKC1ΔXhoKnrTcr Apr; pVK101 carrying pMCC1ΔXho inserted in the EcoRI siteThis study
pVKD1KnrTcr Apr; pVK101 carrying pMCD1 inserted in theEcoRI siteThis study
pRK2013Knr; conjugative helper plasmid 11
pET11aApr; E. coli expression vector 7
pET11R-11Apr; pET11a carrying a 1,045-bp NdeI-BamHI fragment spanningcbbR This study
R. sphaeroides strains
 HRWild type; Smr 44
 1312 cbbR::Tpr 16
 CACChemoautotrophic-competent variant of R. sphaeroides HR 31
E. coli strains
 JM109 47
 MM294 11
 BL21(DE3)Expression strain carrying an IPTG-inducible T7 RNA polymerase gene 35