Table 1.

E. coli strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference or source
Strains
 A5922C600tolA1 (stop after codon 400)recA54
 JC207P4XtolA207 (stop after codon 126)18
 JC864P4X tolB864 (stop after codon 22)J. C. Lazzaroni
 JC34171292 tolB (stop after codon 329)25
 JC77521292 tolB(stop after codon363)Δpal5
 JC77821292 tolA (stop after codon 40)J. C. Lazzaroni
 JC80311292 ΔtolRA13
 JC89311292ompA::TnlacZJ. C. Lazzaroni
 JC8963JE5505ompA::TnlacZ9
 JE5505JE5506 lpp550856
 KS303KS272 lpp550853
 LG10SR1458 tolAThis study
 SR22CA8000htrM378::Tn547
 SR1458MC4100φ (htrA::lacZ)48
 SR3205MC4100surA::ΩKan43
 SC44tolC::Tn559
 SC44tolAtolC::Tn5 tolAC. Wandersman
 TPS13GM1 tolQ13 (stop after codon 36)54
 TPS300GM1tolR::ΩCm55
Plasmids
 pAmpBpBR328 vector, tolBAmprThis study
 pARTolApACYC184 vector,tolA His6 CmrThis study
 pAX617pACYC184 vector, tolCCmr24
 pBPpBR328 vector,tolB pal Tetr5
 pNNpUC9 vector, tolAAmpr2
 pQRApT7-5 vector,orf1 tolQRA Ampr13
 pTPS304pUC9 vector, tolQRAmpr54
 pTPS306pUC9 vector,tolRA Ampr54