Table 2.

Complementation of secretion defects by His-tagged EpsL and EpsM

StrainPlasmid-encoded proteinFraction of toxin B subunit in:
MediumCells
TRH7000(wt)a/pWD6150.830.17
Mut8 (epsL)/pWD6150.070.93
Mut8/pWD615/pMS46 (His)6EpsL0.310.69
Mut8/pWD615/pMS48EpsL(His)6 0.380.62
PU3 (epsM)/pWD6150.220.78
PU3/pWD615/pMMB606EpsM(His)6 0.770.23
  • a wt, wild type with regard to Eps function.