Table 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or relevant phenotypeSource or reference
S. typhimurium
 14028sWild typeATCCa
 CS01914028s phoN2 zxx::6251 Tn10d-Cm 34
 CS401CS019 Strr; wild-type invasion/TTSSS. I. Miller lab
 CJ010CS019 withsirA::Tn10d-Tc 20
 IB040CS019 with prgH::TnphoA, invasion defective 3
 DAP3IB040 withsirA::Tn10d 20
 CJ022DAP3 with pCJ20 20
 JLR020DAP3 with pWKSHRV2.3This work
 JLR002DAP3 with pCJ20aThis work
 JLR010DAP3 with pCJ20bThis work
 JLR016DAP3 with pCJ20cThis work
 JLR018DAP3 with pCJ20dThis work
 CJ023DAP3 with pCJ22 20
 HRB002DAP3 with pCJ22aThis work
 HRB003DAP3 with pCJ22bThis work
 HRB004DAP3 with pCJ22cThis work
 HRB005DAP3 with pCJ22dThis work
 HRB090ΔsirB 1.2-kb in-frame deletion in CS401This work
 CS015 phoP102::Tn10d-Cm PhoP 35
 CS022 pho-24 PhoPc, PhoP-repressed genes constitutively repressed 35
 VV341 hilA::kan 2
 JLR158CS401 with hilA::kanfrom VV341This work
 JLR028 sirC::luc in CS401, measures sirC expression in the presence of SirCThis work
 JLR077CS015 withsirC::luc from JLR028This work
 JLR076CS022 with sirC::lucfrom JLR028This work
 JLR027JLR028 withhilA::kan from VV341This work
 JLR040CJ010 with sirC::lucfrom JLR028This work
 HRB094HRB090 withsirC::luc from JLR028This work
 JLR053ΔsirC 748-bp in-frame deletion in CS401This work
 CL87 iagB::lacZY measureshilA expression in the presence of HilA, wild-type invasivenessGift of C. Lee
 JLR129CS401 withiagB::lacZY from CL87This work
 JLR130JLR053 with iagB::lacZYfrom CL87This work
 JLR151JLR129 withsirA::Tn10d from CJ010This work
 JLR152JLR130 with sirA::Tn10dfrom CJ010This work
 EE638 sspC::Tn5-lacZY 1
 EE637 invF::Tn5-lacZY 1
 EE656 prgH::Tn5-lacZY 2
 JLR138CS401 withsspC::Tn5-lacZY from EE638This work
 JLR135CS401 withinvF::Tn5-lacZY from EE637This work
 JLR136CS401 withprgH::Tn5-lacZY from EE656This work
 JLR141JLR138 with hilA::kanfrom VV341This work
 JLR149JLR135 withhilA::kan from VV341This work
 JLR140JLR136 with hilA::kanfrom VV341This work
 JLR147JLR141 with pCJ20This work
 JLR150JLR149 with pCJ20This work
 JLR145JLR140 with pCJ20This work
 JLR153JLR141 with pCJ13dThis work
 JLR155JLR149 with pCJ13dThis work
 JLR156JLR140 with pCJ13dThis work
Plasmids
 pWSK29Ampr, low-copy-number cloning vector 9
 pWKS30Ampr, low-copy-number cloning vector 9
 pWKSHRV2.3Ampr, 2.3-kbHindIII-EcoRV fragment (contains all oforgA)Gift of C. Lee and V. Bajaj
 pCJ20Ampr, contains 4.4 kb of sirCregion 20
 pCJ20a-dAmpr (see Fig. 1)This work
 pCJ22Ampr, contains 4.6 kb of sirB region 20
 pCJ22a-dAmpr (see Fig. 1)This work
 pCJ13dAmpr, contains sirA expressed from its own promoter 20
 pGPL01Ampr, luciferase suicide vector 17
 psirC::luc Ampr, 1-kb HindIII-NruI fragment from pCJ20 in pGPL01This work
 pKAS32Ampr Strs, pGP704-based suicide vector 39
 pΔsirC Ampr Strs, used to create deletion of 748 bp of sirC This work
 pΔsirB Ampr Strs, used to create deletion of 1.2 kb of sirB ORF1 and ORF2This work
  • a ATCC, American Type Culture Collection.