Table 1.

Bacterial strains and plasmids

Strain or plasmid	Relevant characteristics^a	Source or reference
Strains
P. putida
G7	Wild-type strain containing the NAH7 catabolic plasmid	3
G7.C1	NAH7-cured derivative of G7	D. T. Gibson
G7 Y1	nahY::Km mutant	This work
E. coli
DH5α	F⁻λ⁻ recA1Δ(lacZYA-argF)U169 hsdR17 thi-1 gyrA96 supE44 endA1 relA1 φ80lacZΔM15	Gibco-BRL
S17-1	thi pro hdsR hdsM⁺ recAchromosomal insertion of RP4-2(Tc::Mu Km::Tn7)	14
Plasmids
pBBR1-MCS2	Km^r; multicopy broad-host-range vector	9
pBBR1-MCS5	Gm^r; multicopy broad-host-range vector	9
pLAFR1	Tc^r; low-copy-number broad-host-range cosmid	4
pSUP102::Gm	Gm^r Cm^r; suicide vector in Pseudomonas	13
pUC19	Ap^r; ColE1 replicon	19
pHG59	Km^r; pBBR1-MCS2 containing a 5.9-kbEcoRI fragment from NAH7^b	This work
pHG60	Km^r; pBBR1-MCS2 containing a 4-kbSmaI-EcoRI fragment from pHG59 cloned intoHincII-EcoRI sites	This work
pHG70	Ap^r; the insert of pHG60 is cloned into theKpnI-BamHI sites of pUC19, resulting inEcoRI sites on either side of the insert	This work
pHG95	Ap^r Km^r; pHG70 with the 3′ end of nahY removed with NsiI and replaced with a GenBlock Km^r cassette from Pharmacia that had been digested with PstI	This work
pHG97	Km^rGm^r; the EcoRI insert of pHG95 (containingnahY::Km) cloned into pSUP102::Gm	This work
pHG100	Tc^r; pLAFR1 containing a 25-kb EcoRI fragment from NAH7^b	5
pHG125	Gm^r; 1.7-kb BsiWI fragment from pHG59 that contains nahY introduced into theAcc651 (KpnI) site of pBBR1-MCS5	This work

↵a Ap^r, ampicillin resistant; Gm^r, gentamicin resistant; Cm^r, chloramphenicol resistant, Tc^r, tetracycline resistant.
↵b See Fig. 1.