Table 1.

Strains used in this study

MicroorganismStrainGenotypeSource
E. coliDH5αF′K-12 Δ(lacZYA-argF)U169 supE44 thi1 recA1 endA1 hsdR17 gyrA relA1 (φ80lacZΔM15) F′23
S. cerevisiaeL5366MATa/MATα ura3Δ52/ura3Δ5233
YPH499MATa ura3 leu2 his3 trp1 lys2 ade2M. Gustin
JBY10MATa ura3 leu2 his3 trp1 lys2 ade2 hog1-Δ1::TRP1M. Gustin
W303MATα ura3 leu2 his3 trp1 ade2 can1M. Gustin
J134MATα ura3 leu2 his3 trp1 ade2 can1 hog1::LEU2M. Gustin
C. albicansSC5314Wild type20
CAl4ura3Δ::imm434/ura3Δ::imm43418
RM100ura3Δ::imm434/ura3Δ::imm434his1Δ::hisG/his1Δ::hisG-URA3-hisG54
RM1000ura3Δ::imm434/ura3Δ::imm434his1Δ::hisG/his1Δ::hisG54
CNC11ura3Δ::imm434/ura3Δ::imm434his1Δ::hisG/his1Δ::hisG HOG1/hog1::hisG-URA3-hisG67
CNC12ura3Δ::imm434/ura3Δ::imm434his1Δ::hisG/his1Δ::hisG HOG1/hog1::hisG67
CNC13ura3Δ::imm434/ura3Δ::imm434his1Δ::hisG/his1Δ::hisG hog1::hisG-URA3-hisG/hog1::hisG67
CNC15ura3Δ::imm434/ura3Δ::imm434his1Δ::hisG/his1Δ::hisG hog1::hisG/hog1::hisGThis work
CNC15-10ura3Δ::imm434/ura3Δ::imm434his1Δ::hisG/his1Δ::hisG hog1::hisG/hog1::hisG LEU2/leu2::HOG1 URA3This work
CNCH1ura3Δ::imm434/ura3Δ::imm434his1Δ::hisG/HIS1 hog1::hisG/hog1::hisG LEU2/leu2::HOG1 URA3This work