Table 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionReference
Strains
 ORS571 A. caulinodans wild-type strain 11
 5795ORS571 ethyl methanesulfonate mutant; NifFix 10
 57601ORS571fixK mutant; NifFix 22
 57592ORS571fixL mutant; NifFix 19
 57593ORS571fixJ mutant; NifFix 19
 CA430ORS571phbC::Ω-Spcr This work
 CA439ORS571phbC::Ω-Spcr This work
 SAM100 R. etli phaC::Ω-Kmr 9
Plasmids
 pΔ10.59 R. etli phaCgeneThis laboratory
 pRS1022ORS571 nifA gene expressed constitutively from Kmrpromoter 18
 pLRSA2ORS571nifA gene expressed from its own promoter 36
 pRS2002TranslationalnifH-lacZ fusion 19
 pRS2010Transcriptional nifA-lacZfusion 18
 pRS2028TranscriptionalnifAΔ-lacZ fusion 20
 pRS2004Translational fixK-lacZfusion 22
 pRS3012TranscriptionalfixN-lacZ fusion 29