Table 1.

Analysis of polymorphism at four distinct loci in different fosmids

FosmidaPatternb
16S RNAc16S-23S spacerd16S-GSATeDNA Pole
HaeIIIRsaIHaeIIIAvaII
101G10AAAAAA
60A5BBBBBB
15A5BBbb
43H4AAA
60H6AAa/bB
69H2AAA
87F4Bba/b
C1H5AAAA
C4H1AAAA
C4H9AAAAAB
C7D4AAAAAA
C8B8BBBBBb
C15A3AAAA
C17D2BbBBb
C20B5AAaa/b
  • a The first seven fosmids were isolated from a first library; the last eight fosmids (prefix C) are from a second library.

  • b A or B, pattern identical to that of either 101G10 (=A) or 60A5 (=B); a or b, pattern similar to that of either A or B (see Materials and Methods); —, not determined. Fosmids C1H5, C4H1, C15A3, and C20B5 did not yield PCR products with polymerase (Pol)-specific primers.

  • c Partial sequence (101G10 through 87F4) or RFLP analysis (C1H5 through C20B5).

  • d Partial sequence.

  • e RFLP analysis of PCR products.