Table 1.

Bacterial strains

StrainDescriptionaSource or reference
P. aeruginosa
 PAO1 (K767)bPrototroph
 OCR1PAO1 (K767) nalB21
 K784Spontaneous Smr derivative of OCR136
 K1170K784 ΔmexAB-oprMThis study
 PAO1 (K867)b, cPrototroph; CmrB. H. Iglewski
 K1171Spontaneous Smr derivative of K867This study
 K1168K1171nalBThis study
 K1169K1168::ΔmexAB-oprMThis study
 PDO100PAO1 (K867) ΔrhlI::Tn5013
 PAO-JP1PAO1 (K867) ΔlasI::tetB. H. Iglewski
E. coli
 DH5αendA hsdR17 supE44 thi-1 recA1 gyrA relA1Δ(lacZYA-argF)U169 deoR[φ80dlacΔ(lacZ)M15]1
 S17-1thi pro hsdR recATra+41
  • a Cmr, chloramphenicol resistant; Tra+, mobilizes nonconjugativemob-carrying vectors.

  • b Two different PAO1 strains were used in this study and are distinguished by the laboratory designations K767 and K867.

  • c This PAO1 strain displays substantial resistance to chloramphenicol, in contrast to PAO1 strain K767.