Table 2.

Regulation of the pasABC genes in E. coli CSH50-Iq containing the pas-lacZreporter construct, pP2H

Coresident plasmidAvg β-galactosidase activitya (Miller units) ± SD% Activity
pACYC184 (control)252 ± 6100
pKmM012 ± 15
pKmM231 ± 412
pKmM314 ± 16
pKmM1del1185 ± 7073
pKmM1del2206 ± 982
pTac-pasA-pACYC10 ± 14
pTac-pasB-pACYCNAb NA
pTac-pasC-pACYC226 ± 3790
pTac-pasAB-pACYC2 ± 11
pTac-pasABC-pACYC3 ± 21
  • a β-Galactosidase activity was measured three times on each of three independently selected colonies.

  • b No assay possible because of the lethality of the PasB toxin in CSH50-Iq.