Table 1.

Different classes of Fix, K-antigen-deficient mutants have distinctive phage sensitivity profilesa

Parental or mutant strain or classGenotypeSensitivitybto:
φM1φM5φM6φM7φM9φM10φM11φM12φM12H1φM14φM19φ16-3
Parental strain AK631Wild typeRRRRRRRR+++RR+++
Mutants
rkp-1 rkpA RR++++++++++++++++++++++++R
rkpI RR++++++++++++++++++++++++R
rkpJ RR++++++++++++++++++++++++R
 Class I rkp-085 RRR+++R++++++++++++R
rkp-143 RRR+++R++++++++++++R
rkp-162 RRR+++R+++++++++++++R
rkp-163 RRR++++++++++++++++R
rkp-180 RRR+++R++++++++++++++R
rkp-181 RRR+++R++++++++++++R
 Class II rkp-132 RRR++++R+++++++R++R
rkp-133 RRR++++R+++++++R++R
rkp-135 RRR++++R+++++++++R++R
rkp-161 RRR++++R++++++++R++R
rkp-172 RRR++++R+++++++R++R
 Class III rkp-205 RRR+++++++++++++R
 Class IV rkp-063 RRR+RR++++++++R++R
  • a AK631 is resistant to all phage except φ16-3 and φM12H1. Mutations in genes located in therkp-1 region, rkpA674, rkpI634, andrkpJ671, rendered AK631 sensitive to almost all phages tested with the exception of φM1 and φM5. Mutants in groups I, II, III, and IV also showed increased sensitivities to most phages tested. The mutants from the rkp-1 region and from groups I, II, III, and IV all show distinctive profiles of phage sensitivity and resistance.

  • b Symbols: R, strain resistant to phage; +++, phage forms clear spot on bacteria; ++, phage will form slightly turbid spot on bacteria; +, phage will form a very turbid spot on bacteria and forms plaques with a reduced efficiency.