Table 2.

Measure of competitive inhibition in A. tumefaciens strains with 100 nM 3-oxo-C8-HSL

Inhibitorβ-Galactosidase activity ina:
WCF47(pCF372) with analog at concn (nM):WCF47(pCF372)(pCF218) with analog at concn (nM):
102103104102103104
None340NANA1,400NANA
A3602501501,6001,3001,200
B3502401101,5001,5001,300
C340110.91,6001,5001,200
D2101204701,5001,5001,100
F28038741,3001,2001,000
G2502001801,4001,4001,000
H360320171,8001,500850
I4003303801,4008401,300
J350350971,6001,6001,200
K380340461,5001,600940
L180192.41,4001,200950
M956.05.71,6001,5001,200
N370932.61,4001,3001,100
O410330171,6001,4001,500
P2209.53.61,5001,5001,300
Q350991.91,4001,4001,000
R3203303301,6001,6001,500
S3803202601,6001,6001,400
T330320761,4001,600890
U3401703.91,5001,3001,100
V3702508.61,5001,500780
W3603601401,4001,3001,100
X3403303101,5009901,300
Y3603503401,6009301,700
Z3703903001,6001,1001,500
AA3203702101,3001,5001,100
AB23048331,3001,400940
AC3803703001,4001,500950
AD3403102701,6001,2001,500
AE340220131,6001,500970
AF3603503201,6001,500970
AG370350641,4001,1001,000
  • a The indicated analogs were provided at the noted concentrations to cultures of WCF47(pCF372) containing or lacking pCF218 in AT broth supplemented with 2 mg of opine per ml. Bacteria were cultured for 12 h and assayed for β-galactosidase specific activity (29). β-Galactosidase expression in the absence of autoinducers was less than 2 Miller units. NA, not applicable.