Table 5.

Adaptation in BHI and effects of gad andadiA mutations on AR at pH 2

StrainPertinent genotype% Survival after adaptationa
BHIBHIG
EK227Wild type2065
EK198adiA7055
EF333gadC0.0030.8
EF337adiA gadC0.0020.003
  • a Cells were grown to stationary phase in the media indicated. The cells were diluted to 1 × 106 to 3 × 106 CFU/ml in acid challenge media (pH 2 LB). The results presented indicate survival after 1 h.