Table 1.

Strains and plasmids used in this study

Strain or plasmidGenotype or descriptionSource or reference
E. coliDH5αFφ80dlac ΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK mK ) supE44 λ thi-1 gyrA96 relA1 Bethesda Research Laboratory
P. aeruginosa
  PAO1Wild typeD. Haas
  PAO1214 aotJ::Tn5-751insertion mutant of PAO122
  PAO501 argR; gentamicin resistant22
  PAO4447 leu; kanamycin sensitiveY. Itoh
  PAO4449 aotO::aph mutant of PAO4447This study
  PAO4450 aot::aph mutant of PAO4447; kanamycin resistantThis study
 pUC119 bla lacZ′ cloning vector27
 pUC19 bla lacZ′ cloning vector31
 pQF52 bla lacZ translational fusion vector23
 pST500 aotJ::lacZtranslational fusion of pQF52This study
 pST26360-bp PCR fragment of the aot promoter region cloned intoSmaI site of pUC19This study
 pMO011644Cosmid clone carrying the aot-argR and aruoperons of P. aeruginosa 13
 pYJ6110-kb insert in pMO011644 subcloned intoHincII site of pUC119This study
 pYJ62Similar to pYJ61, but with the opposite orientationThis study
 pTnL1Clone of PAO1214 carrying the aotJ regulatory region and the aph gene of Tn5-751 This study