Table 2.

PCR primers used in this study

Primer	Nucleotide sequence	Position (5′ end)
RR301	5′ `GAG AAC GGA TTC AAC GCC ACC` 3′	348 bp left of attB1-F1^a
RR303	5′ `ACT GCA GCA GAG CAC GCC GTT CG` 3′	523 bp right of attB1-F1^a
RR315	5′ `TGC TCT CAG TTC CCG CAT CC` 3′	clcelement, 209 bp from left end, directed inwards^a
RR316	5′ `GAT GAC GTT GTG ACG ACT GC` 3′	clcelement, 178 bp from left end, directed outwards^a
RR316a	5′ `TGC TGG GTG TGG GTC TAT GGA TGC` 3′	clcelement, 147 bp from left end, directed outwards^a
RR319	5′ `TCA GGC TCA GGC AGT CGT TGT CG` 3′	clcelement, 329 bp from right end, directed outwards^a
RR327	5′ `CTC TCA GTT CCC GCA TCC GCT TCC` 3′	clcelement, 212 bp from left end, directed inwards^a
RR330	5′ `CTG AGC GGG ACA TAT GAC GAG CA` 3′	int-B13, nt 249–271 (Fig. 1), introduction of NdeI site at ATG start codon
RR331	5′ `GAC CCA GGC ATC GAA GAC GT` 3′	int-B13, nt 608–627 (Fig. 1)
RR332	5′ `CAT GAT TAC GAA TTC GCG AGC TCC` 3′	pUC28 polylinker

^a Relative to the positions of exact junctions between the integrated clc element and chromosomal DNA in strain F1.