Table 1.

Bacterial strains and phage used in this study

Strain or phageRelevant genotypeConstruction, source, or reference
Bacterial strains
 JM83 araΔ(lac-pro) thi rpsL (φ80 ΔlacZΔM15) 32
 DF214 his pgi::Mu Δ(zwf-edd)1 eda-1 rpsL D. Fraenkel via E. P. Kennedy
 PT227F thr-1 leuB6Δ(gpt-proA)62 hisG4 argE3 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 mdoB214::Tn10 rpsL31 glnV44 16
 NFB732JM83mdoB214::Tn10 JM83 + P1 (PT227)
 NFB734DF214mdoB214::Tn10 DF214 + P1 (PT227)
 NFB1864JM83 mdoB214::Tn10 mdoC1::Tn5 Tn5 mutant
 NFB1897JM83 mdoB214::Tn10 mdoC1::Tn5 NFB732 + P1 (NFB1864)
 NFB1919DF214mdoC1::Tn5 NFB732 + P1 (NFB1864)
 NFB1933DF214 mdoB214::Tn10 mdoC1::Tn5 NFB734 + P1 (NFB1864)
Bacteriophage
 Phage λNK467 b221 rex::Tn5 cI857 Oam29 Pam80 N. Kleckner via A. Pugsley