Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsPhenotype or relevant markerSource or comment
Strains
Staphylococcus aureus
  SAM13RN4220 (r m+)S. Arvidson (28)
  SAM238325-4 (r+ m+)S. Arvidson (37)
  NT372SAM23yycF1TSUV-induced mutant
  SAM970NT372 purA571::Tn917lac yycF+Emr CmrThis work
  SAM1010SAM23 purA571::Tn917lac yycF1Emr Cmr TSThis work
  SAM1011SAM23 purA571::Tn917lac yycF+Emr CmrThis work
  SAM1061SAM13/pMP782CmrThis work
  SAM1156SAM13 DUP[yycFG, tetK, yycF::ermC]EmrTcrpMP626 integrant
  SAM1157SAM13 DUP[yycFG, tetK, yycG::ermC]EmrTcrpMP705 integrant
  SAM1158SAM13yycF::ermC/pMP782EmrCmrThis work
  SAM1159SAM13yycG::ermC/pMP782EmrCmrThis work
  SAM1443SAM13 DUP[yycFG, tetK, yycFG]TcrpMP1008 integrant
Escherichia coli DH5αFendA1 hsdR17(rKmK+) recA1 gyrA96 relA1Life Technologies, Inc./pUC host strain
Plasmids
 pGEM3Zf(+)Cloning vectorAprPromega
 pLTV1Source of Tn917lacEmrCmrP. Youngman (8)
 pT181Source oftetKTcrS. Arvidson (35)
 pMIN164pE194 (ermC) ligated into pBR325EmrG. A. Bohach (20)
 pC194S. aureus plasmid (cat)CmrBacillus Genetic Stock Center (19)
 pMP16S. aureus-E. coli shuttle vectorEmrThis work
 pMP373NT372-complementing cloneEmrThis work
 pMP621pGEM3Zf(+) containingyycFGAprThis work
 pMP626pMP621 (yycF::ermC, yycG, tetK)Apr Emr TcrThis work
 pMP705pMP621 (yycF, yycG::ermC, tetK)AprEmr TcrThis work
 pMP749Clone recovered from SAM970AprThis work
 pMP782Rescue plasmidAprCmrThis work
 pMP1008pMP621 (tetK)Apr TcrThis work