Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference(s) and/or source
H. ducreyi
 35000Isolated in Manitoba, Canada 23, 24
 R018Isolated in Nairobi, KenyaMichelle Alfa, 6
 181Isolated in Nairobi, KenyaMichelle Alfa, 6
 CA173Isolated in CaliforniaMichelle Alfa,6
 WPB506Isolated in CaliforniaMichelle Alfa, 6
 BG411Isolated in Nairobi, KenyaMichelle Alfa, 6
 041Isolated in SwedenAllan Ronald, 42
 CIP542Isolated in FranceMichelle Alfa, 6
 1145Isolated in Amsterdam, The NetherlandsAllan Ronald
 1151Isolated in GambiaAllan Ronald
 Cha-IIsolated in Texas 42
 Hd12Isolated in Korea 36
 A77Isolated in FranceMichelle Alfa, 6
 6VIsolated in GeorgiaMichelle Alfa, 6
 E1673Isolated in SwedenMichelle Alfa, 6
 78226Isolated in Manitoba, CanadaMichelle Alfa,6
E. coli
 DH5αHost strain for pUC19-based H. ducreyi35000 genomic library constructed with Sau3AI fragments 50
 RR1Host strain for pBR322-based H. ducreyi 35000 genomic library constructed with PstI fragments 50
 XL1-BlueHost strain used for cloning and fusion protein inductionStratagene
Plasmids
 pBluescript II KS(+)Cloning vector; Ampr Stratagene
 pBR322Cloning vector; AmprTetr 50
 pGEX4T-2GST fusion protein vector; Ampr Pharmacia
 pRSETBHis fusion protein vector; Ampr Invitrogen
 pUC19Cloning vector; Ampr 50
 pCW103pGEX4T-2 containing a 997-nt portion of lspA1 (nt 6165 to 7161)a This study
 pCW106pBR322 with a 7.5-kb PstI insert containing the 3′ portion oflspA1 (nt 8179 to 15696)a This study
 pCW107pBR322 with an 8.5-kb PstI insert containing the 3′ portion of lspA2 (nt 8154 to 16697)b This study
 pCW113pBluescript II KS(+) with a 5.9-kbHindIII insert containing a central portion oflspA2 (nt 3367 to 9329)b This study
 pCW114pBluescript II KS(+) with the 8.5-kb insert from pCW107This study
 pCW118pBluescript II KS(+) with a 5.7-kbClaI fragment containing the 5′ portion of lspA2(nt 1 to 5706)b This study
 pCW125pRSETB containing a 459-nt portion of lspA1(nt 5225 to 5683)a This study
 pCW141pGEX4T-2 containing a 534-nt portion oflspA2 (nt 5120 to 5653)b This study
 pDad-5pBR322 with an 8.1-kb PstI insert containing the 5′ portion of lspA1 (nt 1 to 8178)a This study
 pJL13-1pUC18 with a 3.4-kb Sau3AI insert containing a central portion oflspA1 (nt 3130 to 6536)a This study
  • a Nucleotides are numbered in accordance with the lspA1 locus deposited in GenBank; this same numbering scheme is depicted in Fig. 1A.

  • b Nucleotides are numbered in accordance with the lspA2 locus deposited in GenBank; this same numbering scheme is depicted in Fig. 1B.