Table 2.

Fragment sizes resulting from double digestion ofP. putida KT2440 DNAa

FragmentFragment sizeb (kb) obtained with SwaI + PmeINameFragment size (kb) obtained with PmeI + PacI
SwaI-A920PP11,390
SP1680PP21,115
SP2560 PmeI-E678
SwaI-E533PP3548
SP3450PP4510
SwaI-G395PP5446
SP4390PP6384
SwaI-H352PP7337
SwaI-I325 PmeI-F180
SP5320 PacI-E155
SP6172 PacI-F132
SP7165 PacI-G/PP878c
SP8130 PmeI-H19
SwaI-K′105 PmeI-I19
SwaI-L* 65c
SP960
SwaI-M55
SP1045
SP11/SwN30*
SwaI-N′29
SP1221
SwaI-O20
PmeI-H18
PmeI-I18
SP138
SP141
 Estimated total genome size (kb)5,9776,068
  • a The fragments were designatedSwaI, PmeI, or PacI to indicate that the corresponding SwaI, PmeI, or PacI fragment was not cut by SwaI, PmeI, orPacI, respectively. The fragments resulting from the cut of a SwaI or PmeI fragment by the other enzyme are designated SP followed by a number (1 to 14) such that the higher the number, the smaller the DNA fragment. Accordingly, fragments were designated PP followed by a number when they resulted from the digestion of a PmeI fragment by PacI and vice versa.

  • b Average of at least three independent gels. Standard deviations are in the range of 1 to 5% of the given values.

  • c The band is a doublet.