Table 3.

GlutDH activity of a wild-type strain and GlutDH mutants in nutrient broth mediuma

MediumGlutDH activity ofa:
SMY (wild type)BB1268 (ΔgudB::tet)BB1267 (ΔrocG:: ble)BB1271 (ΔrocG::ble ΔgudB::tet)BB1284 (ΔrocG::ble gudB1)
DS (nutrient broth)346440≤45≤45447
DS (nutrient broth) + 1% glucose≤45≤45≤45≤45250
  • a Cells of the indicated strains were grown in DS medium to mid-exponential growth phase (optical density at 600 nm of 0.6 to 0.8). GlutDH activity was assayed and expressed in units as described in Materials and Methods. All numbers are averages of at least two experiments, and the mean errors did not exceed 30%.