Table 1.

Accumulation of lipid II in Tn10cam insertion mutants defective in the synthesis of lipid III

StrainRelevant genotypeAccumulation of lipid IIa
PND257Parent
PND788 wecF::Tn10cam +
PND789 rmlA ECA::Tn10cam +
PND790 wecE::Tn10cam +
  • a +, >1,000 cpm of [3H]GlcNAc-labeled lipid II was recovered from extracts prepared from approximately 108 cells. −, [3H]GlcNAc-labeled lipid II was not detectable in extracts prepared from approximately 108 cells.