Table 1.

NMR data for serrawettin W2 in dimethyl sulfoxide- d6

Residue and position(s)δcfδH(JH,H, Hz)
d-Leu (1)
 NH7.82 (8.8)
 Cαa
 Cβ24.8d
 Cγ23.41.64 m
 Cδc
 Cδc
l-Ser (2)
 NH8.08 (8.2)
 Cαa
 Cβ61.33.63 (11.0, 6.4), 3.56 (11.0, 6.8)
l-Thr (3)
 NH8.03 (8.2)
 Cα58.54.11 (8.4, 2.9)
 Cβ65.44.24 m
 Cγ20.20.97 (6.4)
d-Phe (4)e
 NH7.46 (7.0)
 Cαa
 Cβ37.23.14 (13.7, 4.5), 2.89 (13.7, 7.2)
l-Ile (5)
 NH8.49 (6.4)
 Cα57.63.76 (8.2, 6.8)
 Cβ34.71.77 m
 Cγ40.4d
 Cδc
 Cδc
3-Hydroxy-decanoyl
 C-239.02.64 (14.5, 3.0), 2.36 (14.5, 5.9)
 C-371.84.92b m
 C-431.11.53 br m
 C-5–C-91.23 br
 C-10c
  • a —, the α protons of Phe, Ser, and Leu appear at δ 4.48 to 4.32 as an unresolved multiplet (m).

  • b Partly obscured by the water resonance at δ 4.83 br.

  • c —, five unresolved methyl resonances appear at δ 0.86 to 0.78.

  • d —, the Leuβ and Ileγ protons appear as overlapping multiplets at δ 1.45 to 1.34, and the Leuβ′ and Ileγ′ protons appear as overlapping multiplets at δ 1.18 to 1.09.

  • e The aromatic protons appear as an unresolved multiplet at δ 7.16 to 7.05, and the aromatic carbons appear at δ C2′, 6′, 3′ and 5′ 128.1, 129.1; C1′ 126.5; and C4′ 136.8

  • f The six carbonyl carbon signals appear at δ 172.1, 171.3, 170.6, 170.6, 169.5, and 168.8.