Table 1.

Fragment sizes of all single restriction enzyme digests of SwaI, PmeI, I-CeuI, andPacI of the P. putida KT2440 chromosome

FragmentFragment sizea(kb) obtained with:
SwaIPmeII-CeuIPacI
A9202,5062,8501,875
B7021,0251,2301,750
C6207789461,428
D584729610678
E533674330155
F470170126132
G395b 78
H35219
I32518
J180
K115
K′105
L65b
M55
N30
N′29
O20
 Estimated total genome size (kb)5,9605,9976,0926,018
  • a Average of at least 10 independent gels. Standard deviations were in the range of 1 to 5% of the given value.

  • b The band is a doublet.